6K8B
The open state of RGLG1 VWA domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97889 |
| Spacegroup name | P 41 |
| Unit cell lengths | 136.180, 136.180, 56.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.961 - 2.210 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.19000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.962 | 2.270 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.150 | 1.106 |
| Number of reflections | 52083 | 3843 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 13.7 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M magnesium chloride , 0.1 Msodium chloride, 10%(w/v) PEG1500, 5%(w/v) ethanol |
