6K8A
RGLG1 VWA domain with MIDAS is occupied by water
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978897 |
| Spacegroup name | P 41 |
| Unit cell lengths | 136.280, 136.280, 55.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.009 - 2.400 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.010 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.217 | 1.780 |
| Number of reflections | 39684 | 2909 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.9 | 99.4 |
| Redundancy | 13.5 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M magnesium chloride, 0.1 M sodium citrate ph 6.5, 5%(w/v) MPD |
