6K89

The closed state of RGLG1 VWA domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978897
Spacegroup name	P 21 21 21
Unit cell lengths	45.619, 72.935, 80.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.038 - 1.689
R-factor	0.19
Rwork	0.188
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.143	0.067	1.412
Rmeas	0.149	0.070	1.504
Rpim	0.042	0.020	0.507
Total number of observations	367235
Number of reflections	30477	1682	1463
<I/σ(I)>	2.5
Completeness [%]	99.7	99.8	99.3
Redundancy	12	11.9	8.3
CC(1/2)		0.997	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M NaAc, 25%PEG4K, 8% isopropanol