6K86
The closed state of RGLG1 mutant-E378A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.978897 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.589, 72.555, 80.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.387 - 1.593 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.23270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.081 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
Rmerge | 0.148 | 0.093 | 2.420 |
Rmeas | 0.157 | 0.099 | 2.655 |
Rpim | 0.051 | 0.034 | 1.047 |
Total number of observations | 366686 | ||
Number of reflections | 35438 | 1982 | 1321 |
<I/σ(I)> | 3.5 | ||
Completeness [%] | 96.9 | 99.1 | 73 |
Redundancy | 10.3 | 8.9 | 4.9 |
CC(1/2) | 0.994 | 0.253 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M NaAc, 0.1 M sodium cacodylate ph 6.5, 30% PEG8K |