6K86

The closed state of RGLG1 mutant-E378A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978897
Spacegroup name	P 21 21 21
Unit cell lengths	45.589, 72.555, 80.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.387 - 1.593
R-factor	0.1985
Rwork	0.197
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.148	0.093	2.420
Rmeas	0.157	0.099	2.655
Rpim	0.051	0.034	1.047
Total number of observations	366686
Number of reflections	35438	1982	1321
<I/σ(I)>	3.5
Completeness [%]	96.9	99.1	73
Redundancy	10.3	8.9	4.9
CC(1/2)		0.994	0.253

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M NaAc, 0.1 M sodium cacodylate ph 6.5, 30% PEG8K