6K83
Structure of RGLG1 mutant-D207G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978897 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 141.120, 42.180, 243.440 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 46.513 - 2.390 |
| R-factor | 0.2086 |
| Rwork | 0.205 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.358 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.010 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.157 | 0.983 |
| Number of reflections | 114068 | 2909 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.7 | 99.5 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Mes ph 6.5, 20%PEG6k |
