6K7U
Crystal structure of beta-2 microglobulin (beta2m) of Bat (Pteropus Alecto)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | OSCILLATION CAMERA |
| Collection date | 2017-05-03 |
| Detector | SDMS |
| Wavelength(s) | 0.97776 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 33.707, 114.641, 57.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.527 - 1.601 |
| R-factor | 0.1958 |
| Rwork | 0.195 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 15045 | 15045 |
| <I/σ(I)> | 28.036 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M BIS-TRIS pH 6.5, 8% w/v Polyethylene glycol monomethyl ether 5000 |
