6K7O
Complex structure of LILRB4 and h128-3 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0401 |
| Spacegroup name | P 62 |
| Unit cell lengths | 187.276, 187.276, 183.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.214 - 3.004 |
| R-factor | 0.2302 |
| Rwork | 0.228 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p2t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.660 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 72345 | 7232 |
| <I/σ(I)> | 15.971 | 2.595 |
| Completeness [%] | 99.7 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M ammonium dihydrogen phosphate, 0.1M Tris pH8.5, 50 % v/v MPD |
