6K7D

Crystal structure of MBPapo-Tim21 fusion protein with a 16-residue helical linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	47.010, 88.840, 119.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.631 - 2.000
R-factor	0.1781
Rwork	0.175
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1peb 2ciu
RMSD bond length	0.007
RMSD bond angle	1.097
Data reduction software	HKL-2000 (714)
Data scaling software	HKL-2000 (714)
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.118
Number of reflections	34759	1709
<I/σ(I)>	10.03	2.84
Completeness [%]	99.9	100
Redundancy	6.3	6.3
CC(1/2)		0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MMT, pH 6.0, 24% PEG 1500 (w/v), 0.01M Betaine hydrochloride (additive)