6K79
Glycerol kinase form Thermococcus kodakarensis, complex structure with substrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-13 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.041, 72.128, 115.994 |
| Unit cell angles | 103.43, 94.96, 99.48 |
Refinement procedure
| Resolution | 46.200 - 2.190 |
| R-factor | 0.18724 |
| Rwork | 0.185 |
| R-free | 0.23776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 109477 | 5423 |
| <I/σ(I)> | 14.42 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Imidazole, PEG1000, Calcium acetate |
