6K77

Crystal structure of AMPPNP bound Ck1a alpha from C. neoformans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	89.283, 95.853, 93.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.927 - 2.400
R-factor	0.2225
Rwork	0.216
R-free	0.28220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lp4
RMSD bond length	0.010
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.930	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.186	1.172
Number of reflections	16004	1550
<I/σ(I)>	10.02
Completeness [%]	99.7
Redundancy	13.5
CC(1/2)	0.996	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2M lithium sulfate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000, 30%(w/v) dextran sulfate sodium salt, 5mM AMPPNP, 5mM MgCl2