6K6U
Crystal structure of the human YTHDC2 YTH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 43 |
Unit cell lengths | 49.520, 49.520, 119.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.069 - 2.270 |
R-factor | 0.1978 |
Rwork | 0.195 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.005 |
RMSD bond angle | 0.769 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.070 | 2.440 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 13234 | 2472 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 99.9 | |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Tacsimate,Tris,PEG 3350 |