6K6S
Structure of RNase J1 from Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-28 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.068, 86.068, 486.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.581 - 2.993 |
| R-factor | 0.2987 |
| Rwork | 0.297 |
| R-free | 0.33740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.868 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.581 | 3.170 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 21554 | 3405 |
| <I/σ(I)> | 19.8 | 1.4 |
| Completeness [%] | 95.4 | 96.2 |
| Redundancy | 6.2 | 7 |
| CC(1/2) | 0.971 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | 10mM Citric acid, 70mM Bis-Tris propane pH 9.3, 20% PEG 3350, 0.1M spermine tetrahydrochloride |
