6K5V
Structure of CSY4 Apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.350, 67.938, 317.823 |
| Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
| Resolution | 46.850 - 2.690 |
| R-factor | 0.2287 |
| Rwork | 0.227 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3enj |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.599 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.850 | 2.780 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rpim | 0.039 | 0.237 |
| Number of reflections | 79616 | 7728 |
| <I/σ(I)> | 12.4 | 2.7 |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 85mM MES pH 6.5, 10%(w/v) PEG 20000 |
