6K5S
Crystal structure of the Helical domain of S. pombe Tbf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.498, 80.639, 77.367 |
| Unit cell angles | 111.45, 94.64, 92.05 |
Refinement procedure
| Resolution | 38.297 - 1.904 |
| R-factor | 0.2237 |
| Rwork | 0.221 |
| R-free | 0.27480 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.089 | 0.767 |
| Rmeas | 0.077 | |
| Rpim | 0.818 | |
| Number of reflections | 65194 | 6438 |
| <I/σ(I)> | 23.68 | 2.432 |
| Completeness [%] | 97.6 | 96.5 |
| Redundancy | 7.6 | 7.4 |
| CC(1/2) | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.2M Ammonium citrate tribasic Ph7.0, 20%PEG3350 |
