6K5M

The crystal structure of a serotonin N-acetyltransferase from Oryza Sativa (Rice)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 43 21 2
Unit cell lengths	66.893, 66.893, 82.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.058 - 1.793
R-factor	0.2256
Rwork	0.225
R-free	0.23420
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.790	1.800
Rmerge	0.098	0.857
Number of reflections	18235	1781
<I/σ(I)>	67.435
Completeness [%]	100.0	100
Redundancy	24.9	26.3
CC(1/2)		0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	2M MgCl2, 18% PEG8000, Tris-HCl, PH8.0