6K3F
Crystal Structure of beta-Arrestin 2 in Complex with CXCR7 Phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.170, 127.910, 206.040 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 46.804 - 2.300 |
| R-factor | 0.2358 |
| Rwork | 0.233 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w0p |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.804 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 162425 | 49820 |
| <I/σ(I)> | 1.31 | |
| Completeness [%] | 99.7 | |
| Redundancy | 1 | |
| CC(1/2) | 0.711 | 0.261 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M Ammonium acetate, 0.1M Bis-Tris pH5.5, 25% (w/v) PEG 3350 |
