6K3A

Crystal structure of human PCNA in complex with DNMT1 PIP box motif.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	141.540, 81.598, 68.105
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	40.840 - 2.300
R-factor	0.2195
Rwork	0.217
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mlo
RMSD bond length	0.005
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.840	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.062	0.664
Number of reflections	34458	3366
<I/σ(I)>	0	1.5
Completeness [%]	99.7	99.9
Redundancy	3.2	3.2
CC(1/2)	0.997	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	1.6M sodium citrate