6K35
Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.340, 145.040, 88.680 |
Unit cell angles | 90.00, 109.66, 90.00 |
Refinement procedure
Resolution | 29.350 - 2.360 |
R-factor | 0.1972 |
Rwork | 0.194 |
R-free | 0.26490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rcn |
RMSD bond length | 0.013 |
RMSD bond angle | 1.419 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2-1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.350 | 2.440 |
High resolution limit [Å] | 2.360 | 2.360 |
Number of reflections | 60694 | 6032 |
<I/σ(I)> | 6.09 | |
Completeness [%] | 99.6 | |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Sodium Fluoride, 0.1M Bis Tris Propane pH 6.5, 20% PEG3350 |