6K2O

Structural basis of glycan recognition in globally predominant human P[8] rotavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	113
Detector technology	CCD
Collection date	2018-12-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97852
Spacegroup name	P 63 2 2
Unit cell lengths	80.454, 80.454, 116.593
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.711 - 2.296
R-factor	0.2049
Rwork	0.203
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jdb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.296	2.300
Rmerge	0.159	0.860
Number of reflections	10510	10510
<I/σ(I)>	22.524	4.889
Completeness [%]	100.0
Redundancy	25.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Sodium acetate trihydrate pH 4.5, 2.0M ammonium sulfate