6K2E
Crystal structure of cas2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-05-14 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 62 |
| Unit cell lengths | 101.313, 101.313, 39.585 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.420 - 2.800 |
| R-factor | 0.2138 |
| Rwork | 0.208 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g4d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.420 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 5885 | 587 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.7 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 280 | 0.02M Magnesium, 16.5% v/v 2-propanol, 0.1M Sodium Cacodylate pH 6.6 |
