6K1Y

Crystal structure of Rhodothermus marinus substrate-binding protein at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	46.544, 48.601, 57.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.200 - 1.900
R-factor	0.1831
Rwork	0.178
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z6v
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.101	0.480
Rmeas	0.107	0.543
Rpim	0.034	0.245
Number of reflections	10511	494
<I/σ(I)>	74.57	4.096
Completeness [%]	95.3	91.3
Redundancy	7.6	4.2
CC(1/2)	0.995	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	HEPES, PEG 3350