6K1K
Human nucleosome core particle with H2A.X S139E variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98.15 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.950, 109.460, 183.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.200 - 2.200 |
| R-factor | 0.2323 |
| Rwork | 0.232 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ut9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.492 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.990 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | |
| Rmeas | 0.082 | |
| Rpim | 0.035 | 0.735 |
| Number of reflections | 109157 | 14522 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 98.6 | 91.2 |
| Redundancy | 5.4 | 3.3 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | potassium chloride, manganese chloride, potassium cacodylate |
