6K1J
Human nucleosome core particle with H2A.X variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98.15 |
| Detector technology | PIXEL |
| Collection date | 2014-10-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.750, 109.940, 180.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.880 - 2.850 |
| R-factor | 0.2659 |
| Rwork | 0.265 |
| R-free | 0.31920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nzd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.413 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.880 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.193 | |
| Rmeas | 0.214 | |
| Rpim | 0.090 | 0.655 |
| Number of reflections | 49843 | 7166 |
| <I/σ(I)> | 5.2 | 1.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.5 | 4.2 |
| CC(1/2) | 0.995 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | Potassium Chloride, Manganese Chloride, Potassium Cacodylate |
