6K1I
Human nucleosome core particle with gammaH2A.X variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98.15 |
| Detector technology | PIXEL |
| Collection date | 2018-09-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.850, 109.420, 181.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.700 - 2.750 |
| R-factor | 0.2192 |
| Rwork | 0.218 |
| R-free | 0.27660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.202 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.700 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.193 | |
| Rmeas | 0.202 | |
| Rpim | 0.059 | 0.779 |
| Number of reflections | 54043 | 7776 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 11.3 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | potassium chloride, manganese chloride, potassium cacodylate |
