6K1C

Crystal structure of EXD2 exonuclease domain soaked in Mg and dGMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	46.304, 73.989, 133.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.151 - 2.450
R-factor	0.2168
Rwork	0.214
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k17
RMSD bond length	0.004
RMSD bond angle	0.917
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.101	0.532
Number of reflections	17599	862
<I/σ(I)>	16.19
Completeness [%]	99.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol, Magnesium chloride, Deoxyguanosine monophosphate