6K1B

Crystal structure of EXD2 exonuclease domain soaked in Mn and dGMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97960
Spacegroup name	P 21 21 21
Unit cell lengths	46.339, 73.571, 131.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.811 - 2.605
R-factor	0.2197
Rwork	0.217
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k17
RMSD bond length	0.004
RMSD bond angle	0.804
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.567
Number of reflections	14231	698
<I/σ(I)>	17.81
Completeness [%]	99.2
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol, Manganese chloride, Deoxyguanosine monophosphate