6K0K
Crystal structure of Escherichia coli pyruvate kinase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.693, 137.300, 139.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.950 - 2.680 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e28 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.730 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.131 | 0.952 |
| Rpim | 0.068 | 0.478 |
| Number of reflections | 63484 | 3135 |
| <I/σ(I)> | 8 | 1.25 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 6% v/v Tacsimate TM pH 7.0, 0.1M HEPES pH 7.2, 8% w/v Polyethylene glycol monomethyl ether 5000 |
