6K08
Crystal structure of REV7(R124A/A135D) in complex with a Shieldin3 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.640, 60.640, 133.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.493 - 2.312 |
| R-factor | 0.2131 |
| Rwork | 0.210 |
| R-free | 0.26240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3abd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.490 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rpim | 0.025 | 0.453 |
| Number of reflections | 13088 | 1283 |
| <I/σ(I)> | 16.7 | 2.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.3 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | sodium citrate tribasic, ammonium sulfate |
