6K08

Crystal structure of REV7(R124A/A135D) in complex with a Shieldin3 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	60.640, 60.640, 133.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.493 - 2.312
R-factor	0.2131
Rwork	0.210
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3abd
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.490	2.390
High resolution limit [Å]	2.310	2.310
Rpim	0.025	0.453
Number of reflections	13088	1283
<I/σ(I)>	16.7	2.4
Completeness [%]	99.9	99.9
Redundancy	9.3	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	sodium citrate tribasic, ammonium sulfate