6K06

Crystal structure of Importin-alpha and phosphomimetic GM130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-14
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 21 21 21
Unit cell lengths	73.734, 79.001, 89.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.750
Rwork	0.172
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iq1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.850
High resolution limit [Å]	1.750	1.750
Number of reflections	50110	7420
<I/σ(I)>	20.7
Completeness [%]	95.1
Redundancy	7.3
CC(1/2)	0.990	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.2M Potassium thiocyante, 20% w/v Polyethylene glycol 3350