6K03

Crystal structure of ceH2A-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 32 2 1
Unit cell lengths	63.839, 63.839, 70.032
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.394 - 2.857
R-factor	0.2366
Rwork	0.231
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k01
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	2.960
High resolution limit [Å]	2.857	2.857
Rmerge		0.680
Number of reflections	3967	3967
<I/σ(I)>	12.15
Completeness [%]	97.5
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG3350