6K00

Crystal structure A of ceNAP1-H2A-H2B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	68.298, 78.724, 169.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.351 - 2.198
R-factor	0.2123
Rwork	0.211
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.351	2.280
High resolution limit [Å]	2.198	2.198
Rmerge		0.827
Number of reflections	46721	46721
<I/σ(I)>	17.74
Completeness [%]	99.9
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG8000