6JZV

Crystal structure of SufU from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.25865
Spacegroup name	P 1
Unit cell lengths	35.320, 54.450, 70.380
Unit cell angles	68.99, 75.86, 72.54

Refinement procedure

Resolution	49.409 - 2.000
R-factor	0.213
Rwork	0.211
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xt5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.409	49.409	2.100
High resolution limit [Å]	2.000	10.000	2.000
Rmerge	0.187	0.146	0.931
Rmeas	0.201	0.154	0.999
Total number of observations	499479
Number of reflections	31156	496	8493
<I/σ(I)>	8.64	19.17	2.65
Completeness [%]	99.5	97.3	99.7
Redundancy	8.033	8.651	8.105
CC(1/2)	0.993	0.995	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	0.15 M potassium thiocyanate, 0.1M MES, 20% w/v PEG1500