6JZI

Structure of hen egg-white lysozyme obtained from SFX experiments under atmospheric pressure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2015-05-13
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 43 21 2
Unit cell lengths	79.110, 79.110, 38.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.268 - 2.000
R-factor	0.1615
Rwork	0.159
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wrb
RMSD bond length	0.005
RMSD bond angle	0.746
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	REFMAC
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.940	2.060
High resolution limit [Å]	1.913	2.000
Number of reflections	8615
<I/σ(I)>	16.8
Completeness [%]	100.0
Redundancy	587
CC(1/2)	0.995	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	28% NaCl, 8% PEG6000, 0.1 M Na-acetate pH 3.0