6JYZ
Crystal structure of endogalactoceramidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-10 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.196, 114.520, 128.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.350 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.16800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j7z |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.400 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.069 | 0.335 |
| Rmeas | 0.075 | 0.436 |
| Rpim | 0.030 | 0.273 |
| Number of reflections | 142709 | 6632 |
| <I/σ(I)> | 23.5 | 4.2 |
| Completeness [%] | 81.3 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M magnesium chloride, 0.1M HEPES/Sodium hydroxide pH 7.5 and 30% (v/v) PEG 400 |
