6JYS

GII.13/21 noroviruses recognize glycans with a terminal beta-galactose via an unconventional glycan binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	113
Detector technology	PIXEL
Collection date	2018-06-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97917
Spacegroup name	P 21 21 21
Unit cell lengths	60.728, 99.695, 221.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.635 - 1.697
R-factor	0.166
Rwork	0.165
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.697	1.700
Number of reflections	144550
<I/σ(I)>	19.652
Completeness [%]	96.8
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 5% polyethylene glycol 3350