6JYR

GII.13/21 noroviruses recognize glycans with a terminal beta-galactose via an unconventional glycan binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	113
Detector technology	PIXEL
Collection date	2018-06-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97917
Spacegroup name	P 1 21 1
Unit cell lengths	60.142, 107.512, 99.538
Unit cell angles	90.00, 98.31, 90.00

Refinement procedure

Resolution	40.959 - 1.501
R-factor	0.1784
Rwork	0.178
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.713
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	197138
<I/σ(I)>	25.444
Completeness [%]	98.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 25% polyethylene glycol 3350