6JYI
Crystal structure of the PadR-like transcriptional regulator BC1756 from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.682, 132.672, 49.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.920 |
| R-factor | 0.20884 |
| Rwork | 0.207 |
| R-free | 0.24672 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.060 | 0.471 |
| Number of reflections | 31084 | 3033 |
| <I/σ(I)> | 43.9 | 3.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | PEG 3350, sodium citrate, ammonium dihydrogen phosphate |
