6JY4

Monomeric Form of Bovine Heart Cytochrome c Oxidase in the Fully Reduced State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	50
Detector technology	CCD
Collection date	2011-07-27
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	149.395, 152.395, 174.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.950
R-factor	0.15492
Rwork	0.152
R-free	0.20454
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b1b
RMSD bond length	0.020
RMSD bond angle	1.938
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	1.920
High resolution limit [Å]	1.900	1.900
Rpim	0.032	0.373
Number of reflections	278336	9391
<I/σ(I)>	28.2	2.2
Completeness [%]	89.5
Redundancy	9
CC(1/2)		0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277	sodium phosphate, PEG 4000, DM