6JXD

Human nucleosome core particle with cohesive end DNA termini

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2017-09-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	105.310, 109.660, 183.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.080 - 2.250
R-factor	0.23975
Rwork	0.239
R-free	0.29225
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nzd
RMSD bond length	0.007
RMSD bond angle	1.496
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.180	2.370
High resolution limit [Å]	2.250	2.250
Rpim	0.030	0.760
Number of reflections	101214	14272
<I/σ(I)>	12.2
Completeness [%]	99.7
Redundancy	11.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	manganese chloride, potassium chloride, potassium cacodylate