6JWP

crystal structure of EGOC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	81.675, 120.547, 323.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 3.200
R-factor	0.2329
Rwork	0.230
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r7w
RMSD bond length	0.003
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.240	3.380
High resolution limit [Å]	3.200	3.200
Rmerge	0.173	1.679
Number of reflections	36895	1853
<I/σ(I)>	10.6
Completeness [%]	68.9
Redundancy	7.7
CC(1/2)	0.997	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	100mM HEPES (pH 7.5), 200mM NaCl, and 12%(w/v) PEG 8000