6JW0

Universal RVD R* accommodates cytosine via water-mediated interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-20
Detector	MAR CCD 130 mm
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	85.836, 87.396, 88.135
Unit cell angles	90.00, 104.12, 90.00

Refinement procedure

Resolution	85.470 - 2.200
R-factor	0.2242
Rwork	0.222
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gjp
RMSD bond length	0.013
RMSD bond angle	1.685
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.470	2.420
High resolution limit [Å]	2.200	2.200
Rmeas	0.077	0.077
Number of reflections	58923
<I/σ(I)>	3.45
Completeness [%]	96.8
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	8-10% PEG3350 (w/v), 10% ethanol and 0.1M MES pH 6.7