6JVY

Crystal structure of RBM38 in complex with single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	79.284, 79.284, 66.388
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.495 - 2.003
R-factor	0.1726
Rwork	0.171
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cqd
RMSD bond length	0.008
RMSD bond angle	0.821
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.056	0.039	1.075
Rmeas	0.060	0.041	1.169
Rpim	0.020	0.014	0.435
Number of reflections	16406	1694	1584
<I/σ(I)>	16.8
Completeness [%]	98.8	96.1	96.6
Redundancy	9.4	9	7.8
CC(1/2)		0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.1M Tris-HCl (pH8.0), 2M ammonium sulfate