6JV1
Crystal Structure of N-terminal domain of ArgZ, C264S mutant, bound to Substrate, an arginine dihydrolase from the Ornithine-Ammonia Cycle in Cyanobacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.346, 87.256, 42.818 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 43.630 - 1.200 |
| R-factor | 0.1724 |
| Rwork | 0.172 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jv0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.180 |
| High resolution limit [Å] | 1.160 | 3.150 | 1.160 |
| Rmerge | 0.093 | 0.081 | 0.747 |
| Rmeas | 0.101 | 0.088 | 0.821 |
| Rpim | 0.038 | 0.034 | 0.335 |
| Number of reflections | 98683 | 5148 | 4750 |
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 97.0 | 99.1 | 93.7 |
| Redundancy | 6.7 | 6.8 | 5.6 |
| CC(1/2) | 0.989 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M KI, 0.1 M MES pH 6.5, 19% PEG 4000 |
