6JUR
MsDpo4-DNA complex 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.953724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.670, 80.240, 210.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.260 - 2.060 |
| R-factor | 0.21122 |
| Rwork | 0.209 |
| R-free | 0.25733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jul |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.417 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.260 | 2.180 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.072 | 0.577 |
| Rpim | 0.032 | 0.256 |
| Number of reflections | 58048 | 8368 |
| <I/σ(I)> | 13.7 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6 |
| CC(1/2) | 0.998 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 2KMME 14%, NaCl 0.2mM, Bis Tris Propane pH 6.0 |
