6JUN
MsDpo4-DNA complex 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1272 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.920, 81.320, 210.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.843 - 2.510 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jul |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.665 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.320 | 2.650 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.117 | |
| Number of reflections | 32439 | 4582 |
| <I/σ(I)> | 9.9 | 1.6 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 6.9 | 6.4 |
| CC(1/2) | 0.997 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 2kMME, Bis Tris Propane ph 6.0, NaCl |
