6JUI

The atypical Myb-like protein Cdc5 contains two distinct nucleic acid-binding surfaces

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	72.780, 76.277, 54.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.139 - 2.402
R-factor	0.2378
Rwork	0.234
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.140	2.488
High resolution limit [Å]	2.402	2.402
Number of reflections	11354
<I/σ(I)>	22.1
Completeness [%]	99.3
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	40% MPD