6JR0

Crystal structure of the human nucleosome phased with 12 selenium atoms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-21
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	99.566, 108.514, 168.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.015 - 2.500
R-factor	0.2228
Rwork	0.221
R-free	0.25330
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.778
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.137	0.738
Rmeas	0.142	0.767
Rpim	0.052	0.289
Number of reflections	64077	4451
<I/σ(I)>	13.8	3.6
Completeness [%]	100.0	100
Redundancy	13.7	13.5
CC(1/2)	0.998	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride