6JQ7
The ligand-free structure of human PPARgamma LBD in the presence of the SRC-1 coactivator peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 131.155, 53.406, 54.011 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| R-factor | 0.22186 |
| Rwork | 0.220 |
| R-free | 0.25652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gto |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.336 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 13063 | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 60% (v/v) Tacsimate (pH 7.0) |
