6JQ5

The structure of Hatchet Ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	40.582, 104.275, 126.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.523 - 2.059
R-factor	0.1947
Rwork	0.193
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jq6
RMSD bond length	0.007
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.000	2.000
Rpim	0.036	0.900
Number of reflections	33380	1867
<I/σ(I)>	31.9
Completeness [%]	99.6
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.10M HEPES, pH 6.8, 2.0M (NH4)2SO4