6JPM
Crystal Structure of Odorant Binding Protein 4 in the Natural Predator Chrysopa pallens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2018-10-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 |
Unit cell lengths | 135.745, 135.745, 29.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.993 - 2.098 |
R-factor | 0.1835 |
Rwork | 0.182 |
R-free | 0.21980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.007 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14rc3_3206: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.993 | 2.160 |
High resolution limit [Å] | 2.098 | 2.098 |
Number of reflections | 16276 | |
<I/σ(I)> | 8.1 | |
Completeness [%] | 99.3 | 94 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 30%(V/V) 2-propanol, 100mM Tris pH 7.5, 33%(W/V) PEG3350 |